Culture Blend Liquid Culture Premix – Formula 15 (250 g, Makes 3+ Gallons)
Culture Blend Liquid Culture Premix – Formula 15 (250 g, Makes 3+ Gallons) is backordered and will ship as soon as it is back in stock.
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Description
Description
The dry version of the broth we make in our own lab, in one pouch. Myco Labs Culture Blend is a pre-measured mix of extra light malt extract, dextrose, soy peptone and our nutritional supplement and mineral blend. Weigh it, dissolve it in hot water, and pressure cook. One 250 g pouch makes over 3 US gallons of liquid culture.
- 250 gnet weight (8.82 oz)
- 3+ gallonsper pouch
- 19.4 gper quart of water
- 30 minat 15 PSI
- Formula 1515 years refined
Why growers use Culture Blend
- The recipe behind our own cultures — Formula 15 is the result of 15 years of making and selling liquid cultures and fine-tuning the broth along the way.
- One pouch instead of four ingredients — no sourcing malt extract, dextrose and peptone separately, and no working out ratios.
- Sugars plus nitrogen — malt extract and dextrose feed fast growth, while soy peptone supplies the nitrogen that sugar-only recipes leave out.
- Consistent batch to batch — one blend and one weight per quart, so the jar you make next month matches the one you made today.
- Goes a long way — a single pouch covers about 12 quart batches or 25 pint batches.
Mixing guide
Measure by weight.
| Water | Culture Blend |
|---|---|
| 1 US pint | 9.7 g |
| 1 US quart | 19.4 g |
| 1 liter | 20.5 g |
| 1 US gallon | 77.5 g |
How to make liquid culture
- Mix — add the measured Culture Blend to the matching amount of hot or boiling water and stir until fully dissolved.
- Filter (optional) — pour the mixture through a paper coffee filter to reduce light malt extract sediment.
- Cool and fill — let the broth cool, then fill your jars, leaving headspace at the top.
- Sterilize — pressure cook for 30 minutes at 15 PSI.
- Cool, then inoculate — once the jars are back to room temperature, add 3–5 cc of spore solution or liquid culture, or a clean agar wedge.
- Incubate at 76–82°F, out of direct light, and swirl or stir regularly to keep growth even.
Expect an amber broth. Culture Blend is malt-based, so finished broth is yellow to light amber rather than water-clear. That is normal. Keep to the 30-minute cook time; running much longer can darken the sugars.
Specifications
| Spec | Detail |
|---|---|
| Brand | Myco Labs |
| Product | Culture Blend Liquid Culture Premix, Formula 15 |
| Net weight | 8.82 oz (250 g) |
| Makes | Over 3 US gallons of liquid culture broth |
| Mix ratio | 19.4 g per US quart of water (77.5 g per US gallon) |
| Ingredients | Extra light malt extract, dextrose, soy peptone, proprietary nutritional supplement and mineral blend |
| Sterilization | 30 minutes at 15 PSI |
| Storage | Tightly sealed, cool and dry; measure with a clean, dry utensil |
| Use | For mushroom cultivation. Not for human consumption. |
| SKU | LPM5 |
Frequently asked questions
How much liquid culture does one pouch make?
One 250 g pouch makes over 3 US gallons of liquid culture broth at the label ratio. That works out to about 12 quart batches or 25 pint batches.
How much Culture Blend do I use per jar?
Measure by weight: 9.7 g per US pint of water, 19.4 g per US quart, or 77.5 g per US gallon. In metric that is about 20.5 g per liter. Use a gram scale rather than a measuring spoon so every batch comes out the same.
What is in Culture Blend?
Extra light malt extract, dextrose, soy peptone, and a proprietary nutritional supplement and mineral blend. It is the dry version of the broth recipe our lab has refined over 15 years of producing liquid cultures.
What color should the finished broth be?
Yellow to light amber. The color comes from the malt extract, so the broth will not be water-clear, and that is normal. It stays transparent enough to see mycelium growing in the jar.
Do I have to filter the broth?
No, filtering is optional. Pouring the dissolved mix through a paper coffee filter before you fill your jars removes light malt extract sediment, which makes new growth easier to spot and keeps the culture easier to draw through a needle.
How long do I sterilize it?
Pressure cook for 30 minutes at 15 PSI. Cooking much longer than you need to can darken the sugars in the broth, so stick to the time on the label, then let the jars cool completely to room temperature before you inoculate.
What can I inoculate the broth with?
Spore solution, an existing liquid culture, or a clean agar wedge. For a jar, 3 to 5 cc of spore solution or liquid culture is all it takes.
How should I store the dry mix?
Keep the pouch tightly sealed in a cool, dry place and always use a clean, dry utensil when measuring. Each pouch carries an expiration date on the back. Culture Blend is for mushroom cultivation and is not for human consumption.
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