Liquid Culture Premix – Formula 15 (250 g, Makes 3+ Gallons)
Liquid Culture Premix – Formula 15 (250 g, Makes 3+ Gallons) is backordered and will ship as soon as it is back in stock.
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Description
Description
The dry version of the broth we make in our own lab, in one pouch. Myco Labs Culture Blend is a pre-measured mix of extra light malt extract, dextrose, soy peptone and our nutritional supplement and mineral blend. Weigh it, dissolve it in hot water, and pressure cook. One 250 g pouch makes over 3 US gallons of liquid culture.
Malt extract, dextrose, soy peptone and our mineral blend, pre-measured in one pouch. Weigh it, dissolve it, pressure cook.
- 250 gNet weight (8.82 oz)
- 3+ gallonsPer pouch
- 19.4 gPer quart of water
- 30 minAt 15 PSI
- Formula 1515 years refined
Why growers use Culture Blend
Malt extract and dextrose feed fast mycelium growth. Soy peptone supplies the nitrogen that sugar-only recipes leave out.
A 250 g pouch makes over 3 US gallons of broth: about 12 quart batches or 25 pint batches. No sourcing four ingredients or working out ratios.
One blend and one weight per quart, so the jar you make next month matches the one you made today.
How to make liquid culture
Four steps from pouch to inoculated jar. All you add is water, jars and a pressure cooker.
1Mix
Add the measured premix to hot or boiling water and stir until fully dissolved.
2Fill
Let the broth cool, then fill your jars, leaving headspace at the top.
3Sterilize
Pressure cook for 30 minutes at 15 PSI. Longer cook times can darken the sugars.
4Inoculate
Once jars are back to room temperature, add 3–5 cc of spore solution or liquid culture, or a clean agar wedge.
Tip: for clearer broth, pour the dissolved mix through a paper coffee filter before filling to cut down on malt sediment. Incubate inoculated jars at 76–82°F, out of direct light, and swirl regularly to keep growth even.
Measure by weight
Three weeks after inoculation
Weighing the premix is what makes every batch match. Spoon measurements drift with how packed the powder is, and so does the strength of your broth. Weigh it, add it to the matching amount of hot water, and the jar you make today comes out like the last one.
- 9.7 g per US pint
- 19.4 g per US quart
- 77.5 g per US gallon
Culture Blend is malt-based, so finished broth is yellow to light amber, not water-clear. That's normal.
Keep to the 30-minute cook time; running much longer darkens the sugars.
Mixing guide
Measure by weight.
| Water | Culture Blend |
|---|---|
| 1 US pint | 9.7 g |
| 1 US quart | 19.4 g |
| 1 liter | 20.5 g |
| 1 US gallon | 77.5 g |
Specifications
| Spec | Detail |
|---|---|
| Brand | Myco Labs |
| Product | Culture Blend Liquid Culture Premix, Formula 15 |
| Net weight | 8.82 oz (250 g) |
| Makes | Over 3 US gallons of liquid culture broth |
| Mix ratio | 19.4 g per US quart of water (77.5 g per US gallon) |
| Ingredients | Extra light malt extract, dextrose, soy peptone, proprietary nutritional supplement and mineral blend |
| Sterilization | 30 minutes at 15 PSI |
| Storage | Tightly sealed, cool and dry; measure with a clean, dry utensil |
| Use | For mushroom cultivation. Not for human consumption. |
| SKU | LPM5 |
Make it yourself or start ready-made
LC Premix Formula 15This product
Premium LC Kit10 oz, ready to inject
Premium XL LC Kit28 oz, ready to inject
QuickCulture Mini3 × 30 ml jars
| LC Premix | LC Kit | XL LC Kit | QuickCulture | |
|---|---|---|---|---|
| Use it for | Making your own broth in volume | One ready-to-use jar | A larger ready-to-use jar | Small test batches |
| Format | Dry mix | Sterile jar | Sterile jar | Three mini jars |
| Quantity | 250 g, makes 3+ gal | 10 oz | 28 oz | 3 × 30 ml |
| You supply | Water, jars, pressure cooker | Spores or culture | Spores or culture | Spores or culture |
Pairs well withJar lids with self-healing ports from our filter lids and injection ports, and spores from Spore Genetics. Ready for grain? Browse all grain spawn.
FAQ
How much liquid culture does one pouch make?
One 250 g pouch makes over 3 US gallons of liquid culture broth at the label ratio. That works out to about 12 quart batches or 25 pint batches.
How much Culture Blend do I use per jar?
Measure by weight: 9.7 g per US pint of water, 19.4 g per US quart, or 77.5 g per US gallon. In metric that is about 20.5 g per liter. Use a gram scale rather than a measuring spoon so every batch comes out the same.
What is in Culture Blend?
Extra light malt extract, dextrose, soy peptone, and a proprietary nutritional supplement and mineral blend. It is the dry version of the broth recipe our lab has refined over 15 years of producing liquid cultures.
What color should the finished broth be?
Yellow to light amber. The color comes from the malt extract, so the broth will not be water-clear, and that is normal. It stays transparent enough to see mycelium growing in the jar.
Do I have to filter the broth?
No, filtering is optional. Pouring the dissolved mix through a paper coffee filter before you fill your jars removes light malt extract sediment, which makes new growth easier to spot and keeps the culture easier to draw through a needle.
How long do I sterilize it?
Pressure cook for 30 minutes at 15 PSI. Cooking much longer than you need to can darken the sugars in the broth, so stick to the time on the label, then let the jars cool completely to room temperature before you inoculate.
What can I inoculate the broth with?
Spore solution, an existing liquid culture, or a clean agar wedge. For a jar, 3 to 5 cc of spore solution or liquid culture is all it takes.
How should I store the dry mix?
Keep the pouch tightly sealed in a cool, dry place and always use a clean, dry utensil when measuring. Each pouch carries an expiration date on the back. Culture Blend is for mushroom cultivation and is not for human consumption.
