Pioppino Black Poplar Liquid Culture Syringe (10cc)
Pioppino Black Poplar Liquid Culture Syringe (10cc) is backordered and will ship as soon as it is back in stock.
Couldn't load pickup availability
Description
Description
Pioppino Black Poplar Liquid Culture Syringe (10cc) — Cyclocybe aegerita, first-generation (G1) isolated genetics from Myco Labs. Pioppino is the chef's mushroom of this catalog: dark brown convex caps on slender cream stems, a meaty bite, and a flavor that runs nutty and peppery with a faint floral note when it hits a hot pan.
- SpeciesC. aegerita
- Volume10cc
- GeneticsG1 Isolated
- DifficultyBeginner
- Colonization16–20 Days
Why Growers Choose Pioppino
Pioppino is grown for flavor first. Where most gourmet mushrooms are mild and take on whatever you cook them in, this one arrives with its own character — mildly sweet, peppery, earthy, and noticeably floral in the aroma as it cooks. It is a staple in Italian kitchens for exactly that reason, and it holds up in pasta and hearty sautés where a delicate mushroom would disappear.
The texture is the other half of it. The stems are firm and crisp, closer to asparagus than to a typical mushroom stalk, and they stay that way through cooking. Whole clusters go in the pan stem and all.
Practically, it is an easy grow with an unusually wide tolerance — it fruits anywhere from 55 to 75°F, which is broader than nearly anything else here and means it will produce year-round in a room that drifts with the seasons.
What's In the Package
- One 10cc liquid culture syringe, sealed and lab-tested
- 16-gauge 1.5" luer lock needle
- Sterile alcohol prep pad
Pioppino Growing Specifications
| Specification | Details |
|---|---|
| Scientific Name | Cyclocybe aegerita |
| Also Known As | Pioppino, Black Poplar, Velvet Pioppini, Piopparello |
| Recommended Substrates | Oats, milo, millet or rye berries for spawn; supplemented sawdust for bulk |
| Incubation Temperature | 60–75°F |
| Incubation Humidity | 80–85% |
| Colonization Time | 16–20 days |
| Fruiting Temperature | 55–75°F — one of the widest ranges we carry |
| Fruiting Humidity | 90–93% |
| Time to Harvest | 18–21 days after fruiting conditions begin |
| Spore Drop | Heavy and dark brown — harvest before caps flatten |
| Syringe Volume | 10cc |
| Storage | Refrigerate at 35–45°F; do not freeze |
How to Use Your Liquid Culture Syringe
- Let the syringe come to room temperature, then shake gently to redistribute the mycelium evenly through the broth.
- Flame-sterilize the needle until it glows orange and let it cool for a few seconds.
- Wipe the injection port on your grain spawn bag or jar with the alcohol prep pad.
- Inject 2–3cc per quart jar, or 3–5cc per 3–5 lb spawn bag. One 10cc syringe inoculates roughly 2–4 containers.
- Incubate at 60–75°F in the dark for sixteen to twenty days, shaking once at roughly 30% colonization.
- Spawn to supplemented hardwood sawdust, then introduce fresh air and fruit anywhere in the 55–75°F range.
Frequently Asked Questions
What does Pioppino taste like?
More assertive than most cultivated mushrooms — mildly sweet, peppery and earthy, with a faint floral aroma that comes out as it cooks. The caps are meaty and the stems stay crisp, closer to asparagus in texture. It works particularly well in pasta, risotto and hearty sautés where a milder mushroom would be lost.
Why is there brown dust all over my fruiting chamber?
Pioppino drops a heavy load of dark brown spores, and once the caps flatten out they will coat everything nearby. Harvest while the caps are still convex and slightly curled under to keep the mess down and get better texture. If you are growing in a tent or shelf unit, expect to wipe surfaces between flushes.
What temperature does Pioppino need?
It has one of the widest fruiting ranges in this catalog at 55–75°F, so it suits a room that drifts with the seasons rather than a tightly controlled one. Incubate at 60–75°F. That flexibility makes it a good year-round culture if you only want to keep one strain running.
What substrate should I use for Pioppino?
It is unusually flexible on spawn grain — oats, milo, millet and rye berries all work well. For bulk, supplemented hardwood sawdust gives the best results, since this is a wood-loving species that grows on poplar and other hardwoods in the wild.
How much liquid culture do I use per bag or jar?
Use 2–3cc per quart grain jar and 3–5cc per 3–5 lb spawn bag. A single 10cc Pioppino syringe will inoculate roughly two to four containers. Adding more than the recommended dose does not speed colonization meaningfully and simply uses up the syringe faster.
Do I need a flow hood or still air box to use liquid culture?
A still air box is strongly recommended and a laminar flow hood is better still. Liquid culture syringes ship sealed and lab-tested, but the moment you pierce an injection port you are exposing your grain to whatever is in the room air. Working in still air with flame-sterilized tools dramatically improves your success rate.
How should I store the syringe before use?
Keep it refrigerated between 35 and 45°F and never freeze it — freezing ruptures the cell walls and kills the culture. Store it in the sealed bag it shipped in, away from strong odors. Bring it back to room temperature and shake gently before injecting.
Packed to Arrive Alive
A liquid culture syringe is living mycelium, and the trip from our Illinois lab to your door is the part of its life you have the least control over. That is why every Myco Labs liquid culture ships inside a foil-lined insulated mailer rather than a plain envelope. The insulation buffers the syringe against summer heat sitting in a delivery truck and against sub-freezing temperatures in winter, so your culture arrives viable in either extreme.
When it arrives: move the syringe to the refrigerator at 35–45°F until you are ready to inoculate. Never freeze it. Bring it back to room temperature and shake gently before use.
Let customers speak for us
I've been using these jars for years now with a very high success rate. But I've also had good success with their substrates and pretty much everything I've gotten from this store. Thank you Midwest Grow Kits
I buy most of my supplies here when needed and they never disappoint. If I have an issue they are quick to respond and its typically my fault hahaha.
When I saw my first pin, my universe opened up. mind blowing hobby!
Love it. Easy to put together. Holds a lot of jars, trays, bags, totes, ect. I'm going to get another one for fruiting.
Red sorghum sprouts offers higher antioxidant levels, easier digestion, and rich plant nutrients. Sprouting red sorghum improves its health value by increasing beneficial compounds and reducing barriers to absorbing its nutrients.
Mycelium shot through the casing layer in 2 days. GtPlat. 2/1
Outstanding materials at the lowest cost I could find. Would not pass up this offer if you're serious about growing
This definitely does the job and it’s an excellent product for the price. It took be about 2 hours to set it up and make all the necessary modifications. The FAN solution that comes with it is at best temporary but it will due the job until you can save up for a better fan.
I plan to make tinctures for the messed up nerves in my arms, and other chronic pain bs. Don't want to support too many opportunistic companies, so I just chose the source and be patient. Holistic care and responsibility is a game of patience and understanding. I appreciate your services! Be well, do good! Try to waste less. Oh, the small black gloves are useless unless you have the hands of a small child.😅
Just stirred it, don't worry🫠 no stir bars or magnets have ever been needed here.
Awesome growth 10 days after inoculation. Very happy! Would recommend this priduct and this company!
Working good. Lights my closet just enough for the environment I need! Thanks!
It's started good. Let's hope it continues to grow so I can break a shake it!
Bought the 3-Pack Premium Quick-Colonizing 5-grain Jar (32oz) and inoculated 9 days ago. Love it! Colonization quick and clean. Highly recommend!
So when i got this i was worried because of past experiences. Leave it up to midwest to be nice a patient with me. The team at bonsai gives great support . This flow hood is meant to run a little higher than normal clean room standards because most people need this working from home . This thing is nice and laminar. Very inform . With a speed contoller i average about .45 m/s across the face. It passed everyone one of my agar grid tests . Which blows my mind becuase i ran it through three diffrent 30 mins agar grid tests . Ive used a few flow hoods and this one is my favorite . Perfect for any home or small buisness.
"Upgraded to the MycoClimate 44Q and the difference in consistency is night and day. The dual-chamber water-bath heating keeps temperatures rock-solid without drying out the substrate like standard heating mats do, and the automated HEPA air pump takes all the stress out of fresh air exchange—even tucked away inside a closet.
