Chestnut Mushroom Liquid Culture Syringe (10cc)
Chestnut Mushroom Liquid Culture Syringe (10cc) is backordered and will ship as soon as it is back in stock.
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Description
Description
Chestnut Mushroom Liquid Culture Syringe (10cc) — Pholiota adiposa, first-generation (G1) isolated genetics from Myco Labs. Chestnuts fruit in tight clusters of glossy tan caps on slender stems, and they keep coming back — this is one of the most dependable multi-flush strains we carry, and one of the easiest to recommend to someone on their second or third grow.
- SpeciesP. adiposa
- Volume10cc
- GeneticsG1 Isolated
- DifficultyBeginner
- Colonization~18 Days
Why Growers Choose Chestnut
Chestnut earns its place on reliability rather than drama. It colonizes rye berries readily, tolerates a range of bulk substrates, and — the part that matters — produces flush after flush from the same block where many species give you one good round and quit. For a grower trying to get real food out of a limited amount of substrate, that changes the economics.
It is a cool-weather strain, fruiting anywhere from 50 to 70°F, which puts it alongside Blue Oyster and Shiitake as a fall and winter culture. Once the first tiny primordia appear, the clusters mature in about two to three weeks.
In the kitchen the appeal is texture as much as flavor. The caps are nutty and mild, but the stems stay pleasantly crunchy through cooking, so whole clusters sautéed stem-and-all give you two textures in one pan. They hold their shape far better than an oyster.
What's In the Package
- One 10cc liquid culture syringe, sealed and lab-tested
- 16-gauge 1.5" luer lock needle
- Sterile alcohol prep pad
Chestnut Growing Specifications
| Specification | Details |
|---|---|
| Scientific Name | Pholiota adiposa |
| Recommended Substrates | Rye berries for spawn; supplemented sawdust or straw for bulk |
| Incubation Temperature | 65–75°F |
| Incubation Humidity | 80–85% |
| Colonization Time | ~18 days |
| Fruiting Temperature | 50–70°F |
| Fruiting Humidity | 90–93% |
| Time to Harvest | ~16 days; 2–3 weeks from first primordia |
| Flush Behavior | Reliably multi-flush — expect several rounds per block |
| Syringe Volume | 10cc |
| Storage | Refrigerate at 35–45°F; do not freeze |
How to Use Your Liquid Culture Syringe
- Let the syringe come to room temperature, then shake gently to redistribute the mycelium evenly through the broth.
- Flame-sterilize the needle until it glows orange and let it cool for a few seconds.
- Wipe the injection port on your grain spawn bag or jar with the alcohol prep pad.
- Inject 2–3cc per quart jar, or 3–5cc per 3–5 lb spawn bag. One 10cc syringe inoculates roughly 2–4 containers.
- Incubate at 65–75°F in the dark for around eighteen days, shaking once at roughly 30% colonization.
- Spawn to supplemented sawdust or straw, then drop to 50–70°F and introduce fresh air to trigger pinning.
Frequently Asked Questions
How many flushes will Chestnut produce?
Several, and that is the main reason to grow it. Chestnut is one of the more dependable multi-flush species available, and a well-maintained block will keep producing after the first harvest. Rehydrate the block by soaking it after each flush and return it to fruiting conditions rather than discarding it.
Is Chestnut a good mushroom for beginners?
Yes. It colonizes rye berries readily, is not fussy about bulk substrate, and forgives conditions that would stall a more demanding species. If you have already run an oyster and want to try something with a different texture and flavor without adding complexity, Chestnut is the natural next step.
What temperature does Chestnut need?
Incubate at 65–75°F and fruit at 50–70°F. It genuinely prefers the cooler end of that fruiting range, which makes it a strong autumn and winter strain for an unheated basement or garage. Warm summer rooms are not its friend — pick Pink or Golden Oyster for those months.
How do I cook Chestnut mushrooms?
Cook whole clusters, stems included. The caps are nutty and mild while the stems stay crunchy, so you get two textures at once. Sauté hard in butter or oil until the edges colour, and they hold their shape far better than oyster mushrooms — good in stir-fries where a softer mushroom would collapse.
How much liquid culture do I use per bag or jar?
Use 2–3cc per quart grain jar and 3–5cc per 3–5 lb spawn bag. A single 10cc Chestnut syringe will inoculate roughly two to four containers. Adding more than the recommended dose does not speed colonization meaningfully and simply uses up the syringe faster.
Do I need a flow hood or still air box to use liquid culture?
A still air box is strongly recommended and a laminar flow hood is better still. Liquid culture syringes ship sealed and lab-tested, but the moment you pierce an injection port you are exposing your grain to whatever is in the room air. Working in still air with flame-sterilized tools dramatically improves your success rate.
How should I store the syringe before use?
Keep it refrigerated between 35 and 45°F and never freeze it — freezing ruptures the cell walls and kills the culture. Store it in the sealed bag it shipped in, away from strong odors. Bring it back to room temperature and shake gently before injecting.
Packed to Arrive Alive
A liquid culture syringe is living mycelium, and the trip from our Illinois lab to your door is the part of its life you have the least control over. That is why every Myco Labs liquid culture ships inside a foil-lined insulated mailer rather than a plain envelope. The insulation buffers the syringe against summer heat sitting in a delivery truck and against sub-freezing temperatures in winter, so your culture arrives viable in either extreme.
When it arrives: move the syringe to the refrigerator at 35–45°F until you are ready to inoculate. Never freeze it. Bring it back to room temperature and shake gently before use.
Let customers speak for us
I've been using these jars for years now with a very high success rate. But I've also had good success with their substrates and pretty much everything I've gotten from this store. Thank you Midwest Grow Kits
I buy most of my supplies here when needed and they never disappoint. If I have an issue they are quick to respond and its typically my fault hahaha.
When I saw my first pin, my universe opened up. mind blowing hobby!
Love it. Easy to put together. Holds a lot of jars, trays, bags, totes, ect. I'm going to get another one for fruiting.
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Mycelium shot through the casing layer in 2 days. GtPlat. 2/1
Outstanding materials at the lowest cost I could find. Would not pass up this offer if you're serious about growing
This definitely does the job and it’s an excellent product for the price. It took be about 2 hours to set it up and make all the necessary modifications. The FAN solution that comes with it is at best temporary but it will due the job until you can save up for a better fan.
I plan to make tinctures for the messed up nerves in my arms, and other chronic pain bs. Don't want to support too many opportunistic companies, so I just chose the source and be patient. Holistic care and responsibility is a game of patience and understanding. I appreciate your services! Be well, do good! Try to waste less. Oh, the small black gloves are useless unless you have the hands of a small child.😅
Just stirred it, don't worry🫠 no stir bars or magnets have ever been needed here.
Awesome growth 10 days after inoculation. Very happy! Would recommend this priduct and this company!
Working good. Lights my closet just enough for the environment I need! Thanks!
It's started good. Let's hope it continues to grow so I can break a shake it!
Bought the 3-Pack Premium Quick-Colonizing 5-grain Jar (32oz) and inoculated 9 days ago. Love it! Colonization quick and clean. Highly recommend!
So when i got this i was worried because of past experiences. Leave it up to midwest to be nice a patient with me. The team at bonsai gives great support . This flow hood is meant to run a little higher than normal clean room standards because most people need this working from home . This thing is nice and laminar. Very inform . With a speed contoller i average about .45 m/s across the face. It passed everyone one of my agar grid tests . Which blows my mind becuase i ran it through three diffrent 30 mins agar grid tests . Ive used a few flow hoods and this one is my favorite . Perfect for any home or small buisness.
"Upgraded to the MycoClimate 44Q and the difference in consistency is night and day. The dual-chamber water-bath heating keeps temperatures rock-solid without drying out the substrate like standard heating mats do, and the automated HEPA air pump takes all the stress out of fresh air exchange—even tucked away inside a closet.
