Lion's Mane Liquid Culture Syringe (10cc)
Lion's Mane Liquid Culture Syringe (10cc) is backordered and will ship as soon as it is back in stock.
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Description
Description
Lion's Mane Liquid Culture Syringe (10cc) — Hericium erinaceus, first-generation (G1) isolated genetics from Myco Labs. Lion's Mane is the most recognizable of the tooth fungi, forming shaggy white globes of cascading spines rather than a conventional cap and stem. Our isolate runs aggressively and produces large, full-bodied fruits with dense spine development.
- SpeciesH. erinaceus
- Volume10cc
- GeneticsG1 Isolated
- DifficultyBeginner
- Colonization~10 Days
Why Growers Choose Lion's Mane
Lion's Mane is one of the few gourmet species that is both genuinely easy to colonize and visually spectacular to fruit. The mycelium moves fast — roughly ten days on grain — and it tolerates an unusually wide fruiting window of 50 to 75°F, which means you can run it year-round in most home grow spaces without precise climate control.
In the kitchen it is prized for a texture closer to crab or lobster than to a typical mushroom, holding together well when seared in slabs. It is also one of the most heavily researched functional mushrooms in cultivation, which keeps demand steady among growers producing for tincture and powder rather than the table.
The one thing Lion's Mane will not tolerate is straw. It is a hardwood specialist, and supplemented hardwood sawdust or Master's Mix is where you will see the difference between a modest fruit and a grapefruit-sized one.
What's In the Package
- One 10cc liquid culture syringe, sealed and lab-tested
- 16-gauge 1.5" luer lock needle
- Sterile alcohol prep pad
Lion's Mane Growing Specifications
| Specification | Details |
|---|---|
| Scientific Name | Hericium erinaceus |
| Genetics | First-generation (G1) isolated culture |
| Recommended Substrates | Supplemented hardwood sawdust, Master's Mix, hardwood logs |
| Incubation Temperature | 70–75°F |
| Incubation Humidity | 82–85% |
| Colonization Time | ~10 days |
| Fruiting Temperature | 50–75°F |
| Fruiting Humidity | 90–93% |
| Time to Harvest | ~14 days after fruiting conditions begin |
| Fresh Air Exchange | Moderate — 3–5 exchanges per hour |
| Syringe Volume | 10cc |
| Storage | Refrigerate at 35–45°F; do not freeze |
How to Use Your Liquid Culture Syringe
- Let the syringe come to room temperature, then shake gently to redistribute the mycelium evenly through the broth.
- Flame-sterilize the needle until it glows orange and let it cool for a few seconds.
- Wipe the injection port on your grain spawn bag or jar with the alcohol prep pad.
- Inject 2–3cc per quart jar, or 3–5cc per 3–5 lb spawn bag. One 10cc syringe inoculates roughly 2–4 containers.
- Incubate at 70–75°F in the dark for about ten days, shaking once at roughly 30% colonization to redistribute growth.
- Spawn to supplemented hardwood sawdust or Master's Mix, then cut a single slit in the block and let the fruit form from that one point.
Frequently Asked Questions
How much liquid culture do I use per bag or jar?
Use 2–3cc per quart grain jar and 3–5cc per 3–5 lb spawn bag. A single 10cc Lion's Mane syringe will inoculate roughly two to four containers. Adding more than the recommended dose does not speed colonization meaningfully and simply uses up the syringe faster.
How long does Lion's Mane take to colonize grain?
Roughly ten days at 70–75°F, which makes it one of the faster gourmet cultures we carry. Shaking the jar or bag once at about 30% colonization redistributes the mycelium and can bring that down to seven or eight days.
What is the best substrate for Lion's Mane?
Supplemented hardwood sawdust or Master's Mix. Lion's Mane is a hardwood specialist and performs poorly on straw, unlike oyster species. Master's Mix — typically hardwood sawdust with soy hull supplementation — produces the largest and densest fruits. Hardwood logs also work well for outdoor growers, though fruiting takes considerably longer.
Why is my Lion's Mane growing as a smooth ball with no spines?
That is almost always insufficient fresh air exchange. Lion's Mane forms a smooth, coral-like mass when carbon dioxide accumulates around the fruit, and only develops its characteristic hanging spines once FAE is adequate. Increase air exchange to three to five times per hour while keeping humidity at 90–93% and the spines will begin to differentiate.
Do I need a flow hood or still air box to use liquid culture?
A still air box is strongly recommended and a laminar flow hood is better still. Liquid culture syringes ship sealed and lab-tested, but the moment you pierce an injection port you are exposing your grain to whatever is in the room air. Working in still air with flame-sterilized tools dramatically improves your success rate.
How should I store the syringe before use?
Keep it refrigerated between 35 and 45°F and never freeze it — freezing ruptures the cell walls and kills the culture. Store it in the sealed bag it shipped in, away from strong odors. Bring it back to room temperature and shake gently before injecting.
Is liquid culture better than a spore syringe?
For a known gourmet strain, yes. Liquid culture contains living mycelium from an isolated culture, so it colonizes several days faster than spores and produces genetically consistent fruits every time. Spores must germinate and mate first, which is slower and gives variable results from one grow to the next.
Packed to Arrive Alive
A liquid culture syringe is living mycelium, and the trip from our Illinois lab to your door is the part of its life you have the least control over. That is why every Myco Labs liquid culture ships inside a foil-lined insulated mailer rather than a plain envelope. The insulation buffers the syringe against summer heat sitting in a delivery truck and against sub-freezing temperatures in winter, so your culture arrives viable in either extreme.
When it arrives: move the syringe to the refrigerator at 35–45°F until you are ready to inoculate. Never freeze it. Bring it back to room temperature and shake gently before use.
Sold as a mycological culture for cultivation purposes. Lion's Mane is the subject of ongoing scientific research, but nothing on this page is intended to diagnose, treat, cure, or prevent any disease.
Let customers speak for us
Working good. Lights my closet just enough for the environment I need! Thanks!
It's started good. Let's hope it continues to grow so I can break a shake it!
Bought the 3-Pack Premium Quick-Colonizing 5-grain Jar (32oz) and inoculated 9 days ago. Love it! Colonization quick and clean. Highly recommend!
So when i got this i was worried because of past experiences. Leave it up to midwest to be nice a patient with me. The team at bonsai gives great support . This flow hood is meant to run a little higher than normal clean room standards because most people need this working from home . This thing is nice and laminar. Very inform . With a speed contoller i average about .45 m/s across the face. It passed everyone one of my agar grid tests . Which blows my mind becuase i ran it through three diffrent 30 mins agar grid tests . Ive used a few flow hoods and this one is my favorite . Perfect for any home or small buisness.
"Upgraded to the MycoClimate 44Q and the difference in consistency is night and day. The dual-chamber water-bath heating keeps temperatures rock-solid without drying out the substrate like standard heating mats do, and the automated HEPA air pump takes all the stress out of fresh air exchange—even tucked away inside a closet.
i saw germination in my jar within 2-4 days!! I ordered a 3 pack and i injected 3 different strains and they are all colonizing and germinating very rapidly i see ne difference in rate of germination for genetics they are all moving about the same speed im happy with my purchase!
100% RELIABLE EVERY TIME I LOVE YOU MIDWEST THANL YOU TEAM
Reliable and I know what I am getting. Pretty quick colonization.
I got the grow bag instead of jars this time because I am not interested in the steps of the jars.
Been there done that.
I am not an expert🤓 but now I have enough knowledge and experience to call myself an apprentice.
The thrill of the process is gone. Just give me the dam mushrooms.
I am hurry up and waiting😉.
Seems to be working is all I have to say at this point day 7. The green is reflection not mold.
All I knew was I wanted to grow some mushies for me and the boys, I did a bunch of research on companies and you guys have been around the longest and have the best reviews for these types and I was not disappointed. Took exactly 8 weeks to harvest my first 4 ounces and more on the way! Thanks so much for all the awesome guides and videos! Made it so easy!
It's a great monotub kit. I recommend throwing the horse booster, used the whole bag to 4 small quick grain jars and the 7.5 mix. I learned how to do a pf tek with these guys 10 years ago when you still got the sticky light for the lid and the monotub was a blue tub with polyfill. These monotub kits have come a very long way since then. Substrate has always been clean and performed better than what I try to recreate with a pressure cooker.
10cc liquid culture and follow the instructions mgk gives you hard to mess up.
So far , I only have a little bit on agar , and am only beginning to study this mycelium. Super excited to try to put it to use in this Aspen tree.
Everything is looking real good so far
If your ever in need of replacing your hepa filter on your bonsai flowhood these are an exact match to whichever size you have. Worth noting that a filter will actually work a lot longer than is recommended due to physics but if your externals get dirty or damaged it’s worth replacing.
Shipping was lightning fast
